Fluorescence Staining
1) Can you see the tissue using the fluorescence microscope? Does the tissue appear similar to the first and second slide?
No, we could not see the tissue as a whole. The tissue does not appear similar to the first and second slide.
2) Describe what you observe. How many colours appear when using the fluorescence microscope and what are the colours?
Only one colour appeared—blue.
3) What do you think the colours represent? Why?
We think the colour represents the nucleus of the cells because the Hoescht dye that we used binds with DNA, and DNA is only found in the nucleus of the cells. Hence, we think that the blue colour represents the nucleus of the cells.
4) Can you determine what is/are the pattern of location/distribution of the colours in the tissue? If no, why not?
No, because under the fluorescent microscope, only the parts that are stained are visible. In this case, only the nucleus could be seen because it is the only organelle that is stained. So, we could only see the colour of the stained organelle and not the other parts of the cells in the tissue.
5) Are there any other observations you have identified? If so, describe them.
There were no other observations.
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